One-step, high-yield purification of human prostatic acid phosphatase from seminal fluid by gel-filtration HPLC under nondenaturing conditions.
نویسندگان
چکیده
This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.
منابع مشابه
Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions.
We report a protocol for concomitant purification to homogeneity of both prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] and prostate-specific antigen, from human seminal fluid. The method requires only two chromatographic steps: passage through an Affigel-Blue column and gel filtration HPLC. This is a fast, efficient procedure for purification...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 34 5 شماره
صفحات -
تاریخ انتشار 1988